Journal: PLoS ONE

Article Title: Rectified Cell Migration on Saw-Like Micro-Elastically Patterned Hydrogels with Asymmetric Gradient Ratchet Teeth

doi: 10.1371/journal.pone.0078067

Figure Lengend Snippet: The conditions used to prepare the gels are described in . (a) Phase contrast microscopy image of the gel. Sale bar: 100 µm. (b) Distribution of Young’s modulus in a single unit of the gel. Unit size: 90 µm. Elasticity peak: ca. 500 kPa. (c) Trajectories of 3T3 fibroblasts cultured on gels with PSLs. Cells were observed by time-lapse microscopy for 24 hr and images were acquired at 15 min intervals. (d) Time-course of ensemble averaged trajectories with standard deviation in the presence (red) and absence (blue) of PSLs. n=30 cells.

Article Snippet: The mouse fibroblast cell line (NIH/3T3) was purchased from Dainippon Sumitomo Pharmaceutical Co. Ltd. (Osaka, Japan) and cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco BRL), 3.5 g/l glucose, 2 mM L-glutamine, 50 units/ml penicillin, and 50 μg/ml streptomycin.

Techniques: Microscopy, Cell Culture, Time-lapse Microscopy, Standard Deviation

Journal: Biomicrofluidics

Article Title: Correlation between cell migration and reactive oxygen species under electric field stimulation

doi: 10.1063/1.4932662

Figure Lengend Snippet: The migration rate (a) and directedness (in cosθ) (b) of NIH 3T3 fibroblasts after being stimulated by different strengths of EFs with and without the addition of 1 μM β-lapachone.

Article Snippet: Cell preparation The fibroblast cell line NIH 3T3 was purchased from the Bioresource Collection and Research Center (BCRC), Taiwan.

Techniques: Migration

Journal: Biomicrofluidics

Article Title: Correlation between cell migration and reactive oxygen species under electric field stimulation

doi: 10.1063/1.4932662

Figure Lengend Snippet: (a) Fluorescent and bright-field images of NIH 3T3 fibroblasts after being stimulated by different strengths of EFs with and without the addition of 1 μM β-lapachone. Scale bar = 25 μm. (b) Mean fluorescent intensities of cells in (a).

Article Snippet: Cell preparation The fibroblast cell line NIH 3T3 was purchased from the Bioresource Collection and Research Center (BCRC), Taiwan.

Techniques:

Journal: Biomicrofluidics

Article Title: Correlation between cell migration and reactive oxygen species under electric field stimulation

doi: 10.1063/1.4932662

Figure Lengend Snippet: (a) Fluorescent and bright-field images of NIH 3T3 fibroblasts after stimulation with different strengths of EFs with and without the addition of 20 μg/ml α-tocopherol. Scale bar = 25 μm. (b) Mean fluorescent intensities of cells in (a). The migration rate (c) and directedness (in cosθ) (d) of NIH 3T3 fibroblasts after being stimulated by different strengths of EFs with and without the addition of 20 μg/ml α-tocopherol.

Article Snippet: Cell preparation The fibroblast cell line NIH 3T3 was purchased from the Bioresource Collection and Research Center (BCRC), Taiwan.

Techniques: Migration